| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Karahalis, Alex (10)
|
MAJY |
36.41 |
32.19 |
-4.22 |
| 1 |
Estep, Brandon M (10)
|
MASHIP |
32.94 |
32.63 |
-0.31 |
| 1 |
Bowker, Patrick J (9)
|
MAUN02 |
41.97 |
39.74 |
-2.23 |
| 2 |
Turner, Seth M (10)
|
MAACST |
36.26 |
34.79 |
-1.47 |
| 2 |
Burkholder, Moses L (9)
|
MANHAC |
49.49 |
41.06 |
-8.43 |
| 3 |
Estep, Derek T (9)
|
MASHIP |
37.84 |
35.62 |
-2.22 |
| 3 |
Delgado, Zach J (10)
|
MANHAC |
1:01.68 |
43.90 |
-17.78 |
| 4 |
Ritter, Nicholas C (10)
|
MAEAST |
37.99 |
36.68 |
-1.31 |
| 4 |
Reigner, Benjamin A (10)
|
MANHAC |
45.48 |
45.08 |
-0.40 |
| 5 |
Drake, Matthew S (10)
|
MAUN06 |
40.59 |
38.36 |
-2.23 |
| 5 |
Cohn, Matthew E (9)
|
MAACST |
|
52.15 |
- |
| 6 |
Ludwig, Connor S (10)
|
MANHAC |
40.94 |
38.94 |
-2.00 |
| 6 |
Saylor, Ethan J (10)
|
MANHAC |
1:03.91 |
58.82 |
-5.09 |
| 7 |
Cielecki, Edward (10)
|
MAAAC |
41.09 |
40.30 |
-0.79 |
| 8 |
Lloyd, Ethan J (9)
|
MANHAC |
41.16 |
42.96 |
1.80 |
| 9 |
Troy, William E (10)
|
MAUN06 |
40.28 |
43.14 |
2.86 |
|
Saylor, Braydon P (10)
|
MANHAC |
|
DQ |
- |
|
Gonzalez, Ayden C (9)
|
MANHAC |
|
DQ |
- |
|
Petertonjes, Gabriel A (10)
|
MAACST |
|
DQ |
- |